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primescript reverse transcription rt reagent kit  (TaKaRa)


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    Structured Review

    TaKaRa primescript reverse transcription rt reagent kit
    Primescript Reverse Transcription Rt Reagent Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 2447 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reverse+transcription+kit/PrimeScript+1st+strand+cDNA+Synthesis+Kit/pm42892802-60-20-19
    Average 99 stars, based on 2447 article reviews
    primescript reverse transcription rt reagent kit - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Hepatocyte IER2 attenuates APAP-induced liver injury by regulating ACOT2-mediated lipid metabolism in mice.
    Article Snippet: .. cDNA was prepared with a reverse transcription kit (RR036A; TaKaRa, Kyoto, Japan). .. The qPCR assay was performed using a Roche LC96 RealTime PCR Detection System (Roche, Basel, Switzerland).

    Article Title: Histidine alleviates copper-induced intestinal inflammation through oxidative stress-mediated mitochondrial damage and the Hif1α-Tnfα/Il1β pathway
    Article Snippet: Total RNA was isolated from rainbow trout intestinal tissue and enterocytes with TRIzol reagent (Thermo Fisher Scientific, 10296028), as previously described ( ). .. Complementary DNA (cDNA) was synthesized from the extracted RNA using a Reverse Transcription Kit (TaKaRa, Tokyo, Japan, 6110A). ..

    Article Title: RAD51D deficiency enhances IFN-JAK-STAT signaling to upregulate PD-L1 and increase sensitivity to PD-L1 blockade in colorectal cancer
    Article Snippet: Total RNA was extracted using TRIzol reagent (Invitrogen, 15596018CN). .. Complementary DNA (cDNA) was synthesized using a reverse transcription kit (Takara, RR036A). .. qPCR was performed using SYBR Green Master Mix (Yeasen, 11202ES) on a QuantStudio 7 Flex real-time PCR system (Thermo Fisher Scientific).

    Article Title: Loss of hepatic circadian organization in fatty liver syndrome identifies CLOCK as a regulator of lipid homeostasis in laying hens.
    Article Snippet: A total of 10 μL of CCK-8 reagent was added to each well, followed by incubation for 2 h. Absorbance at 450 nm was measured using a Varioskan LUX microplate reader (Thermo Fisher Scientific, Waltham, MA, USA). .. Reverse transcription and real-time quantitative PCR (qRT-PCR) After total RNA extraction, cDNA was synthesized using a reverse transcription kit (Takara, Cat. No. RR037A) and used as the template for qRT-PCR. .. qRT-PCR was performed using SYBR Green Master Mix (Takara, Cat. No. RR086A), with at least three replicates for each sample.

    Article Title: Hyaluronic Acid Microneedle With α-Boswellic Acid Heal Oral Ulcers by Anti-inflammatory Activity.
    Article Snippet: RNA concentration and purity were measured using a spectrophotometer (NanoDrop 2000c, ABI, USA, 210 nm). .. cDNA was synthesized from 1 μg of total RNA in a 20 μL reaction using a reverse transcription kit (RR047A, TAKARA, Japan). .. For real- time quantitative PCR, each qPCR reaction was set up in a total volume of 20 μL, including 10 μL of 2× qPCR Master Mix (FSQ- 201, TOYOBO, Japan), 1 μL of each primer (10 μM, synthesized by Tsingke Biotechnology, China), 2 μL of cDNA template, and 6 μL of nuclease- free water.

    Article Title: Multi-enzyme supplementation in low-fish meal diets for shrimp: Combined effects of carbohydrase, phytase, and protease on nutrient utilization, growth, gut health, and immunity in Pacific white shrimp (Penaeus vannamei)
    Article Snippet: This study evaluated the combined effects of carbohydrase, phytase, and protease in a low-fish meal (FM) diet on Pacific white shrimp performance.. Nine isonitrogenous (400 g/kg crude protein) and isolipidic (80 g/kg crude lipid) diets were formulated using FM and soybean meal as the principal protein sources, including a positive control (PC; 200 g/kg FM) and a negative control (NC; 100 g/kg FM) diet.. The NC diet supplemented with phytase (100 mg/kg) and alkaline protease (25 mg/kg) was designated as the D3 diet.

    Article Title: LukS-PV targets human C5aR1 to reprogram tumor-associated macrophages and potentiate immunotherapy in hepatocellular carcinoma
    Article Snippet: Total RNA was extracted from cells or tissues using the Quick-RNA Miniprep Kit (R1055, Zymo Research) according to the manufacturer’s instructions. .. Complementary DNA (cDNA) was synthesized from 1 μg of total RNA using a reverse transcription kit (6110 A, Takara). ..

    Article Title: Physiological Response States Distinguish Contrasting Zinc-Mediated Cadmium Redistribution Across 44 Rice Cultivars
    Article Snippet: RNA integrity was verified using agarose gel electrophoresis, and the RNA concentration was measured using a NanoDrop spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). .. First-strand cDNA was synthesized using a reverse transcription kit (6215A; Takara Bio, Shiga, Japan). .. RT-qPCR was performed using a CFX96 Real-Time PCR System (Bio-Rad, Hercules, CA, USA) with SYBR Green Master Mix (B690016; Sangon Biotech, Shanghai, China).

    Synthesized:

    Article Title: Histidine alleviates copper-induced intestinal inflammation through oxidative stress-mediated mitochondrial damage and the Hif1α-Tnfα/Il1β pathway
    Article Snippet: Total RNA was isolated from rainbow trout intestinal tissue and enterocytes with TRIzol reagent (Thermo Fisher Scientific, 10296028), as previously described ( ). .. Complementary DNA (cDNA) was synthesized from the extracted RNA using a Reverse Transcription Kit (TaKaRa, Tokyo, Japan, 6110A). ..

    Article Title: RAD51D deficiency enhances IFN-JAK-STAT signaling to upregulate PD-L1 and increase sensitivity to PD-L1 blockade in colorectal cancer
    Article Snippet: Total RNA was extracted using TRIzol reagent (Invitrogen, 15596018CN). .. Complementary DNA (cDNA) was synthesized using a reverse transcription kit (Takara, RR036A). .. qPCR was performed using SYBR Green Master Mix (Yeasen, 11202ES) on a QuantStudio 7 Flex real-time PCR system (Thermo Fisher Scientific).

    Article Title: Loss of hepatic circadian organization in fatty liver syndrome identifies CLOCK as a regulator of lipid homeostasis in laying hens.
    Article Snippet: A total of 10 μL of CCK-8 reagent was added to each well, followed by incubation for 2 h. Absorbance at 450 nm was measured using a Varioskan LUX microplate reader (Thermo Fisher Scientific, Waltham, MA, USA). .. Reverse transcription and real-time quantitative PCR (qRT-PCR) After total RNA extraction, cDNA was synthesized using a reverse transcription kit (Takara, Cat. No. RR037A) and used as the template for qRT-PCR. .. qRT-PCR was performed using SYBR Green Master Mix (Takara, Cat. No. RR086A), with at least three replicates for each sample.

    Article Title: Hyaluronic Acid Microneedle With α-Boswellic Acid Heal Oral Ulcers by Anti-inflammatory Activity.
    Article Snippet: RNA concentration and purity were measured using a spectrophotometer (NanoDrop 2000c, ABI, USA, 210 nm). .. cDNA was synthesized from 1 μg of total RNA in a 20 μL reaction using a reverse transcription kit (RR047A, TAKARA, Japan). .. For real- time quantitative PCR, each qPCR reaction was set up in a total volume of 20 μL, including 10 μL of 2× qPCR Master Mix (FSQ- 201, TOYOBO, Japan), 1 μL of each primer (10 μM, synthesized by Tsingke Biotechnology, China), 2 μL of cDNA template, and 6 μL of nuclease- free water.

    Article Title: Multi-enzyme supplementation in low-fish meal diets for shrimp: Combined effects of carbohydrase, phytase, and protease on nutrient utilization, growth, gut health, and immunity in Pacific white shrimp (Penaeus vannamei)
    Article Snippet: This study evaluated the combined effects of carbohydrase, phytase, and protease in a low-fish meal (FM) diet on Pacific white shrimp performance.. Nine isonitrogenous (400 g/kg crude protein) and isolipidic (80 g/kg crude lipid) diets were formulated using FM and soybean meal as the principal protein sources, including a positive control (PC; 200 g/kg FM) and a negative control (NC; 100 g/kg FM) diet.. The NC diet supplemented with phytase (100 mg/kg) and alkaline protease (25 mg/kg) was designated as the D3 diet.

    Article Title: LukS-PV targets human C5aR1 to reprogram tumor-associated macrophages and potentiate immunotherapy in hepatocellular carcinoma
    Article Snippet: Total RNA was extracted from cells or tissues using the Quick-RNA Miniprep Kit (R1055, Zymo Research) according to the manufacturer’s instructions. .. Complementary DNA (cDNA) was synthesized from 1 μg of total RNA using a reverse transcription kit (6110 A, Takara). ..

    Article Title: Physiological Response States Distinguish Contrasting Zinc-Mediated Cadmium Redistribution Across 44 Rice Cultivars
    Article Snippet: RNA integrity was verified using agarose gel electrophoresis, and the RNA concentration was measured using a NanoDrop spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). .. First-strand cDNA was synthesized using a reverse transcription kit (6215A; Takara Bio, Shiga, Japan). .. RT-qPCR was performed using a CFX96 Real-Time PCR System (Bio-Rad, Hercules, CA, USA) with SYBR Green Master Mix (B690016; Sangon Biotech, Shanghai, China).

    Real-time Polymerase Chain Reaction:

    Article Title: Loss of hepatic circadian organization in fatty liver syndrome identifies CLOCK as a regulator of lipid homeostasis in laying hens.
    Article Snippet: A total of 10 μL of CCK-8 reagent was added to each well, followed by incubation for 2 h. Absorbance at 450 nm was measured using a Varioskan LUX microplate reader (Thermo Fisher Scientific, Waltham, MA, USA). .. Reverse transcription and real-time quantitative PCR (qRT-PCR) After total RNA extraction, cDNA was synthesized using a reverse transcription kit (Takara, Cat. No. RR037A) and used as the template for qRT-PCR. .. qRT-PCR was performed using SYBR Green Master Mix (Takara, Cat. No. RR086A), with at least three replicates for each sample.

    RNA Extraction:

    Article Title: Loss of hepatic circadian organization in fatty liver syndrome identifies CLOCK as a regulator of lipid homeostasis in laying hens.
    Article Snippet: A total of 10 μL of CCK-8 reagent was added to each well, followed by incubation for 2 h. Absorbance at 450 nm was measured using a Varioskan LUX microplate reader (Thermo Fisher Scientific, Waltham, MA, USA). .. Reverse transcription and real-time quantitative PCR (qRT-PCR) After total RNA extraction, cDNA was synthesized using a reverse transcription kit (Takara, Cat. No. RR037A) and used as the template for qRT-PCR. .. qRT-PCR was performed using SYBR Green Master Mix (Takara, Cat. No. RR086A), with at least three replicates for each sample.



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