primescript reverse transcription rt reagent kit (TaKaRa)
99
Structured Review
TaKaRa
primescript reverse transcription rt reagent kit
Primescript Reverse Transcription Rt Reagent Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 2447 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reverse+transcription+kit/PrimeScript+1st+strand+cDNA+Synthesis+Kit/pm42892802-60-20-19
Average 99 stars, based on 2447 article reviews
Primescript Reverse Transcription Rt Reagent Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 2447 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reverse+transcription+kit/PrimeScript+1st+strand+cDNA+Synthesis+Kit/pm42892802-60-20-19
Average 99 stars, based on 2447 article reviews
primescript reverse transcription rt reagent kit - by Bioz Stars,
2026-11
99/100 stars
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Reverse Transcription:Article Title: Hepatocyte IER2 attenuates APAP-induced liver injury by regulating ACOT2-mediated lipid metabolism in mice. Article Snippet: .. cDNA was prepared with a Article Title: Histidine alleviates copper-induced intestinal inflammation through oxidative stress-mediated mitochondrial damage and the Hif1α-Tnfα/Il1β pathway Article Snippet: Total RNA was isolated from rainbow trout intestinal tissue and enterocytes with TRIzol reagent (Thermo Fisher Scientific, 10296028), as previously described ( ). .. Complementary DNA (cDNA) was synthesized from the extracted RNA using a Article Title: RAD51D deficiency enhances IFN-JAK-STAT signaling to upregulate PD-L1 and increase sensitivity to PD-L1 blockade in colorectal cancer Article Snippet: Total RNA was extracted using TRIzol reagent (Invitrogen, 15596018CN). .. Complementary DNA (cDNA) was synthesized using a Article Title: Loss of hepatic circadian organization in fatty liver syndrome identifies CLOCK as a regulator of lipid homeostasis in laying hens. Article Snippet: A total of 10 μL of CCK-8 reagent was added to each well, followed by incubation for 2 h. Absorbance at 450 nm was measured using a Varioskan LUX microplate reader (Thermo Fisher Scientific, Waltham, MA, USA). .. Reverse transcription and real-time quantitative PCR (qRT-PCR) After total RNA extraction, cDNA was synthesized using a Article Title: Hyaluronic Acid Microneedle With α-Boswellic Acid Heal Oral Ulcers by Anti-inflammatory Activity. Article Snippet: RNA concentration and purity were measured using a spectrophotometer (NanoDrop 2000c, ABI, USA, 210 nm). .. cDNA was synthesized from 1 μg of total RNA in a 20 μL reaction using a Article Title: Multi-enzyme supplementation in low-fish meal diets for shrimp: Combined effects of carbohydrase, phytase, and protease on nutrient utilization, growth, gut health, and immunity in Pacific white shrimp (Penaeus vannamei) Article Snippet: This study evaluated the combined effects of carbohydrase, phytase, and protease in a low-fish meal (FM) diet on Pacific white shrimp performance.. Nine isonitrogenous (400 g/kg crude protein) and isolipidic (80 g/kg crude lipid) diets were formulated using FM and soybean meal as the principal protein sources, including a positive control (PC; 200 g/kg FM) and a negative control (NC; 100 g/kg FM) diet.. The NC diet supplemented with phytase (100 mg/kg) and alkaline protease (25 mg/kg) was designated as the D3 diet. Article Title: LukS-PV targets human C5aR1 to reprogram tumor-associated macrophages and potentiate immunotherapy in hepatocellular carcinoma Article Snippet: Total RNA was extracted from cells or tissues using the Quick-RNA Miniprep Kit (R1055, Zymo Research) according to the manufacturer’s instructions. .. Complementary DNA (cDNA) was synthesized from 1 μg of total RNA using a Article Title: Physiological Response States Distinguish Contrasting Zinc-Mediated Cadmium Redistribution Across 44 Rice Cultivars Article Snippet: RNA integrity was verified using agarose gel electrophoresis, and the RNA concentration was measured using a NanoDrop spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). .. First-strand cDNA was synthesized using a Synthesized:Article Title: Histidine alleviates copper-induced intestinal inflammation through oxidative stress-mediated mitochondrial damage and the Hif1α-Tnfα/Il1β pathway Article Snippet: Total RNA was isolated from rainbow trout intestinal tissue and enterocytes with TRIzol reagent (Thermo Fisher Scientific, 10296028), as previously described ( ). .. Complementary DNA (cDNA) was synthesized from the extracted RNA using a Article Title: RAD51D deficiency enhances IFN-JAK-STAT signaling to upregulate PD-L1 and increase sensitivity to PD-L1 blockade in colorectal cancer Article Snippet: Total RNA was extracted using TRIzol reagent (Invitrogen, 15596018CN). .. Complementary DNA (cDNA) was synthesized using a Article Title: Loss of hepatic circadian organization in fatty liver syndrome identifies CLOCK as a regulator of lipid homeostasis in laying hens. Article Snippet: A total of 10 μL of CCK-8 reagent was added to each well, followed by incubation for 2 h. Absorbance at 450 nm was measured using a Varioskan LUX microplate reader (Thermo Fisher Scientific, Waltham, MA, USA). .. Reverse transcription and real-time quantitative PCR (qRT-PCR) After total RNA extraction, cDNA was synthesized using a Article Title: Hyaluronic Acid Microneedle With α-Boswellic Acid Heal Oral Ulcers by Anti-inflammatory Activity. Article Snippet: RNA concentration and purity were measured using a spectrophotometer (NanoDrop 2000c, ABI, USA, 210 nm). .. cDNA was synthesized from 1 μg of total RNA in a 20 μL reaction using a Article Title: Multi-enzyme supplementation in low-fish meal diets for shrimp: Combined effects of carbohydrase, phytase, and protease on nutrient utilization, growth, gut health, and immunity in Pacific white shrimp (Penaeus vannamei) Article Snippet: This study evaluated the combined effects of carbohydrase, phytase, and protease in a low-fish meal (FM) diet on Pacific white shrimp performance.. Nine isonitrogenous (400 g/kg crude protein) and isolipidic (80 g/kg crude lipid) diets were formulated using FM and soybean meal as the principal protein sources, including a positive control (PC; 200 g/kg FM) and a negative control (NC; 100 g/kg FM) diet.. The NC diet supplemented with phytase (100 mg/kg) and alkaline protease (25 mg/kg) was designated as the D3 diet. Article Title: LukS-PV targets human C5aR1 to reprogram tumor-associated macrophages and potentiate immunotherapy in hepatocellular carcinoma Article Snippet: Total RNA was extracted from cells or tissues using the Quick-RNA Miniprep Kit (R1055, Zymo Research) according to the manufacturer’s instructions. .. Complementary DNA (cDNA) was synthesized from 1 μg of total RNA using a Article Title: Physiological Response States Distinguish Contrasting Zinc-Mediated Cadmium Redistribution Across 44 Rice Cultivars Article Snippet: RNA integrity was verified using agarose gel electrophoresis, and the RNA concentration was measured using a NanoDrop spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). .. First-strand cDNA was synthesized using a Real-time Polymerase Chain Reaction:Article Title: Loss of hepatic circadian organization in fatty liver syndrome identifies CLOCK as a regulator of lipid homeostasis in laying hens. Article Snippet: A total of 10 μL of CCK-8 reagent was added to each well, followed by incubation for 2 h. Absorbance at 450 nm was measured using a Varioskan LUX microplate reader (Thermo Fisher Scientific, Waltham, MA, USA). .. Reverse transcription and real-time quantitative PCR (qRT-PCR) After total RNA extraction, cDNA was synthesized using a RNA Extraction:Article Title: Loss of hepatic circadian organization in fatty liver syndrome identifies CLOCK as a regulator of lipid homeostasis in laying hens. Article Snippet: A total of 10 μL of CCK-8 reagent was added to each well, followed by incubation for 2 h. Absorbance at 450 nm was measured using a Varioskan LUX microplate reader (Thermo Fisher Scientific, Waltham, MA, USA). .. Reverse transcription and real-time quantitative PCR (qRT-PCR) After total RNA extraction, cDNA was synthesized using a |